ZhaoLab - ShanghaiTech University

2025

125. Conformational ensembles for protein structure prediction.

Yang J, Cheng WX, Zhang P, Wu G, Sheng ST, Yang J, Zhao S, Hu Q, Ji W, Shi Q. Sci Rep. 2025 Mar 12;15(1):8513. doi: 10.1038/s41598-024-84066-z. PMID: 40074747; PMCID: PMC11904239.

124. Cryo-EM structure of an activated GPR4-Gs signaling complex. [MEMBRANE PROTEIN]

Ma Y, Wang Y, Tang M, Weng Y, Chen Y, Xu Y, An S, Wu Y, Zhao S, Xu H, Li D, Liu M, Lu W, Ru H, Song G. Nat Commun. 2025 Jan 11;16(1):605. doi: 10.1038/s41467-025-55901-2. PMID: 39799123; PMCID: PMC11724869.

123. Peptide-Scaffolded detergents for membrane protein studies. [MEMBRANE PROTEIN]

Yang M, Dai Y, Zhou F, Zhou X, Qiu Y, Tan Y, Zhao S, Xue D, Zhao F, Tao H. Chemistry. 2025 Mar 17;31(16):e202404520. doi: 10.1002/chem.202404520. Epub 2025 Feb 28. PMID: 39777805.

2024

122. CORD: The chordata olfactory receptor database.

Han W, Bao S, Liu J, Wu Y, Zeng L, Zhang T, Chen N, Yao K, Fan S, Huang A, Feng Y, Zhang G, Zhang R, Zhu H, Hua T, Liu Z, Cao L*, Huang X*, Zhao S*. Protein Cell. 2024 Sep 20:pwae050. doi: 10.1093/procel/pwae050. Epub ahead of print. PMID: 39302986.

121. The versatile binding landscape of the TAAR1 pocket for LSD and other antipsychotic drug molecules. [MEMBRANE PROTEIN]

Jiang K, Zheng Y, Zeng L, Wang L, Li F, Pu J, Lu Y, Zhao S*, Xu F*. Cell Rep. 2024 Jul 23;43(7):114505. doi: 10.1016/j.celrep.2024.114505. Epub 2024 Jul 13. PMID: 39002128.

120. Zero-shot prediction of mutation effects with multimodal deep representation learning guides protein engineering.

Cheng P, Mao C, Tang J, Yang S, Cheng Y, Wang W, Gu Q, Han W, Chen H, Li S, Chen Y, Zhou J, Li W, Pan A, Zhao S, Huang X, Zhu S*, Zhang J*, Shu W*, Wang S*. Cell Res. 2024 Jul 5 doi: 10.1038/s41422-024-00989-2. Epub ahead of print. PMID: 38969803.

119. Novel cocrystal structures of peptide antagonists bound to the human melanocortin receptor 4 unveil unexplored grounds for structure-based drug design. [MEMBRANE PROTEIN]

Gimenez LE, Martin C, Yu J, Hollanders C, Hernandez CC, Wu Y, Yao D, Han GW, Dahir NS, Wu L, Van der Poorten O, Lamouroux A, Mannes M, Zhao S, Tourwé D, Stevens RC*, Cone RD*, Ballet S*. J Med Chem. 2024 Feb 22;67(4):2690-2711. doi: 10.1021/acs.jmedchem.3c01822. Epub 2024 Feb 12. PMID: 38345933.

118. A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways. [MEMBRANE PROTEIN]

Zhang Z, Lin X, Wei L, Wu Y, Xu L, Wu L, Wei X, Zhao S, Zhu X*, Xu F*. Cell Discov. 2024 Jan 5;10(1):3. doi: 10.1038/s41421-023-00627-y. PMID: 38182578; PMCID: PMC10770037.

2023

117. 噬菌体Z基因组生物合成通路的研究进展.

Chen H, Zhao S*. 遗传. 2023 Oct 20;45(10):887-903. doi: 10.16288/j.yczz.23-059. PMID: 37872112.

116. Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness.

Chen S, Wu Y, Gao Y, Wu C, Wang Y, Hou C, Ren M, Zhang S, Zhu Q, Zhang J, Yao Y, Huang M, Qi YB, Liu XS, Horng T, Wang H, Ye D, Zhu Z*, Zhao S*, Fan G*. Cell Rep. 2023 Oct 12;42(10):113246. doi: 10.1016/j.celrep.2023.113246. Epub ahead of print. PMID: 37831605.

115. Chemical-induced phase transition and global conformational reorganization of chromatin.

Wang T, Shi S, Shi Y, Jiang P, Hu G, Ye Q, Shi Z, Yu K, Wang C, Fan G, Zhao S, Ma H, Chang ACY, Li Z, Bian Q*, Lin CP*. Nat Commun. 2023 Sep 9;14(1):5556. doi: 10.1038/s41467-023-41340-4. PMID: 37689690.

114. Structure-based ligand discovery targeting the transmembrane domain of frizzled receptor FZD7. [MEMBRANE PROTEIN]

Li C, Wu Y, Wang W, Xu L, Zhou Y, Yue Y, Wu T, Yang M, Qiu Y, Huang M, Zhou F, Zhou Y, Hao P, Lin Z, Wang MW, Zhao S*, Yang D*, Xu F*, Tao H*. J Med Chem. 2023 Sep 14;66(17):11855-11868. doi: 10.1021/acs.jmedchem.2c01795. Epub 2023 Sep 5. PMID: 37669317.

113. Orthogonally engineered albumin with attenuated macrophage phagocytosis for the targeted visualization and phototherapy of liver cancer.

Dou WT, Qiu P, Shi Y, Zhu L, Guo C, Li N, Zang Y, Liu T, Zhao S, Pan Y, Dong L, Sessler JL*, Tan Y*, Li J*, Wang H*, Tian H, He XP*. J Am Chem Soc. 2023 Aug 9;145(31):17377-17388. doi: 10.1021/jacs.3c05052. Epub 2023 Jul 27.

112. GPCRana: a webserver for quantitative analysis of GPCR structures. [MEMBRANE PROTEIN]

Guo Y, Zhou Q*, Wei B, Wang MW*, Zhao S*. Structure. 2023 Jun 22:S0969-2126(23)00203-4. doi: 10.1016/j.str.2023.06.008. Epub ahead of print.

111. Alternative Z-genome biosynthesis pathway shows evolutionary progression from archaea to phage. [ENZYME FUNCTION AND MECHANISM]

Tong Y, Wu X, Liu Y, Chen H, Zhou Y, Jiang L, Li M*, Zhao S*, Zhang Y*. Nat Microbiol. 2023 Jul;8(7):1330-1338. doi: 10.1038/s41564-023-01410-1. Epub 2023 Jun 12.

110. Structural insights into the human niacin receptor HCA2-Gi signalling complex. [MEMBRANE PROTEIN]

Yang Y, Kang HJ, Gao R, Wang J, Han GW, DiBerto JF, Wu L, Tong J, Qu L, Wu Y, Pileski R, Li X, Zhang XC, Zhao S, Kenakin T, Wang Q, Stevens RC, Peng W*, Roth BL*, Rao Z*, Liu Z-J*. Nat Commun. 2023 Mar 27;14(1):1692. doi: 10.1038/s41467-023-37177-6.

109. Rational design of GDP‑d‑mannose mannosyl hydrolase for microbial l‑fucose production. [ENZYME FUNCTION AND MECHANISM]

Fu C, Xu X, Xie Y, Liu Y, Liu M, Chen A, Blamey JM, Shi J, Zhao S*, Sun J*. Microb Cell Fact. 2023 Mar 24;22(1):56. doi: 10.1186/s12934-023-02060-y.

108. Effects of novel variants in BMP15 gene on litter size in Mongolia and Ujimqin sheep breeds.

Wang Y, Chi Z, Jia S, Zhao S, Cao G, Purev C, Cang M, Yu H, Li X, Bao S, Liu Y, Wang J, Tong B*. Theriogenology. 2023 Mar 1;198:1-11. doi: 10.1016/j.theriogenology.2022.12.005.

107. The activation mechanism and antibody binding mode for orphan GPR20. [MEMBRANE PROTEIN]

Lin X, Jiang S, Wu Y, Wei X, Han GW, Wu L, Liu J, Chen B, Zhang Z, Zhao S, Cherezov V, Xu F*. Cell Discov. 2023 Feb 28;9(1):23. doi: 10.1038/s41421-023-00520-8.

106. 脊索动物嗅觉受体基因命名法的发展. [MEMBRANE PROTEIN]

韩伟, 赵素文*, 黄行许*, 林峰* 生命的化学. [1]韩伟,赵素文,黄行许等.脊索动物嗅觉受体基因命名法的发展[J].生命的化学,2023,43(05):648-656.DOI:10.13488/j.smhx.20230130.

105. Development of Yin-Yang ligand for cannabinoid receptors. [MEMBRANE PROTEIN]

Qiu Y, Zhao Y, Hu T, Yang M, Li F, Li C, Gu W, Yang X*, Zhao S*, Tao H*. Bioorg Chem. 2023 Jan 20;133:106377. doi: 10.1016/j.bioorg.2023.106377. Online ahead of print.

104. Cryo-EM structures of orphan GPR21 signaling complexes. [MEMBRANE PROTEIN]

Lin X, Chen B, Wu Y, Han Y, Qi A, Wang J, Yang Z, Wei X, Zhao T, Wu L, Xie X, Sun J, Zheng J*, Zhao S*, Xu F*. Nat Commun. 2023 Jan 13;14(1):216. doi: 10.1038/s41467-023-35882-w.

2022

103. Transition-linker containing detergents for membrane protein studies. [MEMBRANE PROTEIN]

Luo W, Yang M, Zhao Y, Wang H, Yang X, Zhang W, Zhao F*, Zhao S*, Tao H*. Chemistry. 2022 Dec 9;28(69):e202202242. doi: 10.1002/chem.202202242.

102. A core epitope targeting antibody of SARS-CoV-2. [ANTIVIRUS]

Zhao S, Liu F, Qiu S, Lan Q, Wu Y, Xu W, Ke J, Yang J, Liu X, Wang K, Guo H, Xia S, Zhang F, Wang J, Hu X, Lu L, Jiang S, Zhao S, Liu L*, Xie Y*, Yang X*, Wang H*, Zhong G*. Protein Cell. 2022 Oct 26;14(1):74-78. doi: 10.1093/procel/pwac042.

101. Cytoophidia coupling adipose architecture and metabolism. [ENZYME FUNCTION AND MECHANISM]

Liu J, Zhang Y, Zhou Y, Wang QQ, Ding K, Zhao S, Lu P, Liu JL*. Cell Mol Life Sci. 2022 Oct 1;79(10):534.doi: 10.1007/s00018-022-04567-w.

100. Structural basis for strychnine activation of human bitter taste receptor TAS2R46. [MEMBRANE PROTEIN]

Xu W, Wu L, Liu S, Liu X, Cao X, Zhou C, Zhang J, Fu Y, Guo Y, Wu Y, Tan Q, Wang L, Liu J, Jiang L, Fan Z, Pei Y, Yu J, Cheng J, Zhao S, Hao X, Liu ZJ*, Hua T*. Science. 2022 Sep 16;377(6612):1298-1304. doi: 10.1126/science.abo1633.

99. H2B Lys34 ubiquitination induces nucleosome distortion to stimulate Dot1l activity. [ENZYME FUNCTION AND MECHANISM]

Ai H, Sun M, Liu A, Sun Z, Liu T, Cao L, Liang L, Qu Q, Li Z, Deng Z, Tong Z, Chu G, Tian X, Deng H, Zhao S, Li JB*, Lou Z*, Liu L*. Nat Chem Biol. 2022 Sep;18(9):972-980. doi: 10.1038/s41589-022-01067-7. Epub 2022 Jun 23. PMID: 35739357.

98. Building the chordata olfactory receptor database using more than 400,000 receptors annotated by Genome2OR. [MEMBRANE PROTEIN]

Han W, Wu Y, Zeng L, Zhao S*. Sci China Life Sci. 2022 Dec;65(12):2539-2551. doi: 10.1007/s11427-021-2081-6. Epub 2022 Jun 10. PMID: 35696018.

97. The unconventional activation of the muscarinic acetylcholine receptor M4R by diverse ligands. [MEMBRANE PROTEIN]

Wang J, Wu M, Chen Z, Wu L, Wang T, Cao D, Wang H, Liu S, Xu Y, Li F, Liu J, Chen N, Zhao S, Cheng J*, Wang S*, Hua T*. Nat Commun. 2022 May 23;13(1):2855. doi: 10.1038/s41467-022-30595-y.

96. Ugi reaction mediated detergent assembly for membrane protein studies. [MEMBRANE PROTEIN]

Yang M, Luo W, Zhang W, Wang H, Xue D, Wu Y, Zhao S, Zhao F*, Zheng X*, Tao H*. Chem Asian J. 2022 Aug 1;17(15):e202200372. doi: 10.1002/asia.202200372. Epub 2022 Jun 7. PMID: 35575910.

95. Association analysis between novel variants in LEPR gene and litter size in Mongolia and ujimqin sheep breeds. [ENZYME FUNCTION AND MECHANISM]

Ma S, Ji X, Cang M, Wang J, Yu H, Liu Y, Zhang W, Wu Y, Zhao S, Cao G, Tong B*. Theriogenology. 2022 Apr 15;183:79-89. doi: 10.1016/j.theriogenology.2022.02.014.

94. Identification and characterization of the biosynthetic pathway of the sulfonolipid capnine. [ENZYME FUNCTION AND MECHANISM]

Liu Y, Wei Y, Teh TM, Liu D, Zhou Y, Zhao S, Ang EL, Zhao H*, Zhang Y*. Biochemistry. 2022 Dec 20;61(24):2861-2869. doi: 10.1021/acs.biochem.2c00102. Epub 2022 Apr 12. PMID: 35414181.

93. MD simulations revealing special activation mechanism of cannabinoid receptor 1. [MEMBRANE PROTEIN]

Wu Y, Li X, Hua T, Liu ZJ, Liu H*, Zhao S*. Front Mol Biosci. 2022 Mar 29;9:860035. doi: 10.3389/fmolb.2022.860035.

92. Crystal structure of a constitutive active mutant of adenosine A2A receptor. [MEMBRANE PROTEIN]

Cui M, Zhou Q, Xu Y, Weng Y, Yao D, Zhao S, Song G*. IUCrJ. 2022 Mar 17;9(Pt 3):333-341. doi: 10.1107/S2052252522001907.

2021

91. Structures of the human cholecystokinin receptors bound to agonists and antagonists. [MEMBRANE PROTEIN]

Zhang X, He C, Wang M, Zhou Q, Yang D, Zhu Y, Feng W, Zhang H, Dai A, Chu X, Wang J, Yang Z, Jiang Y, Sensfuss U, Tan Q, Han S,Reedtz-Runge S, Xu EH, Zhao S*, Wang MW*, Wu B*, Zhao Q*. Nat Chem Biol. 2021 Dec;17(12):1230-1237. doi: 10.1038/s41589-021-00866-8.

90. Structure-based design of dual-acting compounds targeting adenosine A2A receptor and histone deacetylase as novel tumor immunotherapeutic agents. [MEMBRANE PROTEIN]

Yan W, Ling L, Wu Y, Yang K, Liu R, Zhang J, Zhao S, Zhong G, Zhao S, Jiang H*, Xie C*, Cheng J*. ChemistryOpen. J Med Chem. 2021 Nov 25;64(22):16573-16597. doi: 10.1021/acs.jmedchem.1c01155.

89. Structure-Activity Relationship Studies of Hydantoin-Cored Ligands for Smoothened Receptor. [MEMBRANE PROTEIN]

Liu Y, Zhou F, Ding K, Xue D, Zhu Z, Li C, Li F, Xu Y, Xu F, Le Z, Zhao S*, Tao H*. ChemistryOpen. 2021 Oct;10(10):1028-1032. doi: 10.1002/open.202100216.

88. Elucidation of distinct modular assemblies of smoothened receptor by bitopic ligand measurement. [MEMBRANE PROTEIN]

Zhao F, Wu Y, Zhou F, Xue D, Zhao S, Lu W, Liu X, Hu T, Qiu Y, Li R, Gu T, Xu Y, Xu F, Zhong G, Jiang Z, Zhao S*, Tao H*. J Med Chem. 2021 Sep 23;64(18):13830-13840. doi: 10.1021/acs.jmedchem.1c01220.

87. Copper(II) gluconate boosts the Anti-SARS-CoV-2 effect of disulfiram in vitro. [ANTIVIRUS]

Xu L, Xu W, Zhao S, Zhao S, Lu L*, Lin BL*. biorXiv. 2021 Sep 17. doi: 10.1101/2021.09.17.460613.

86. Rational remodeling of atypical scaffolds for the design of photoswitchable cannabinoid receptor tools. [MEMBRANE PROTEIN]

Hu T, Zheng G, Xue D, Zhao S, Li F, Zhou F, Zhao F, Xie L, Tian C, Hua T, Zhao S, Xu Y, Zhong G, Liu ZJ, Makriyannis A, Stevens RC, Tao H*. J Med Chem. 2021 Sep 3;64(18):13752-13765. doi: 10.1021/acs.jmedchem.1c01088.

85. A computational evaluation of targeted oxidation strategy. [ANTIVIRUS]

Xu L, Tong J, Wu Y, Zhao S*, Lin BL*. Biophys Chem. 2021 Sep;276:106610. doi:10.1016/j.bpc.2021.106610.

84. Ligands of adrenergic receptors: a structural point of view. [MEMBRANE PROTEIN]

Wu Y, Zeng L, Zhao S*. Biomolecules. 2021 Jun 24;11(7):936. doi: 10.3390/biom11070936.

83. Discovery of novel allosteric modulators targeting an extra-helical binding site of GLP–1R using structure- and ligand-based virtual screening. [MEMBRANE PROTEIN]

Zhou Q, Guo W, Dai A, Cai X, Vass M, de Graaf C, Shui W, Zhao S*, Yang D*, Wang MW*. Biomolecules. 2021 Jun 23;11(7):929. doi: 10.3390/biom11070929.

82. Affinity mass spectrometry-based fragment screening identified a new negative allosteric modulator of the adenosine A2A receptor targeting the sodium ion pocket. [MEMBRANE PROTEIN]

Lu Y, Liu H, Yang D, Zhong L, Xin Y, Zhao S, Wang MW*, Zhou Q*, Shui W*. ACS Chem Biol. 2021 Jun 18;16(6):991-1002. doi: 10.1021/acschembio.0c00899.

81. Protein contact map prediction using multiple sequence alignment dropout and consistency learning for sequences with less homologs.

Liu X#, Jin L#, Gao S*, Zhao S*. bioRxiv. 2021 May 13. doi: 2021.05.12.443740.

80. A widespread pathway for substitution of adenine by diaminopurine in phage genomes. [PATHWAY DOCKING AND GENOME MINING] [ENZYME FUNCTION AND MECHANISM]

Zhou Y#, Xu X#, Wei Y, Cheng Y, Guo Y, Khudyakov I, Liu F, He P, Song Z, Li Z, Gao Y, Ang E. L, Zhao H*, Zhang Y*, Zhao S*. Science. 2021 Apr 30;372(6541):512-516. doi: 10.1126/science.abe4882.

Media coverage:

ZTCG: Viruses expand the genetic alphabet. Science, Perspective | Michael W. Grome, Farren J. Isaacs.

Weird viral DNA spills secrets to biologist. Nature, News | Ewen Callaway.

Some viruses have a mysterious 'Z' genome. Live Science, News | Yasemin Saplakoglu.

Some viruses use an alternative genetic alphabet. The Scientist, News & Opinion | Abby Olena.

Some viruses thwart bacterial defenses with a unique genetic alphabet. Science News, News | Tina Hesman Saey.

Z-基因组的生物合成奥秘被揭示！ 合成生物学, 评论 | 金交羽, 周佳海.

The Shadow Biosphere The Primordial Scoop, Single Scoops | Steven A. Benner.

79. Conjugated secondary 12α-hydroxylated bile acids promote liver fibrogenesis. [MEMBRANE PROTEIN]

Xie G, Jiang R, Wang X, Liu P, Zhao A, Wu Y, Huang F, Liu Z, Rajani C, Zheng X, Qiu J, Zhang X, Zhao S, Bian H, Gao X, Sun B, Jia W., EBioMedicine. 2021 Apr;66:103290. doi: 10.1016/j.ebiom.2021.103290.

78. Large-scale analysis of bioactive ligand conformational strain energy by Ab initio calculation.

Tong J, Zhao S*. J Chem Inf Model. 2021 Mar 22;61(3):1180-1192. doi: 10.1021/acs.jcim.0c01197.

77. G蛋白偶联受体的共同激活机制. [MEMBRANE PROTEIN]

周庆同, 戴之卓, 赵素文*, 自然杂志. 2021 Feb 25;43(1):45-52. doi: 10.3969/j.issn.0253-9608.2021.01.007.

76. G protein-coupled receptors: structure- and function-based drug discovery. [MEMBRANE PROTEIN]

Yang D#, Zhou Q#, Labroska V#, Qin S, Darbalaei S, Wu Y, Yuliantie E, Xie L, Tao H, Cheng J, Liu Q, Zhao S.#; Shui W*, Jiang Y*, Wang MW*. Signal Transduct Target Ther. 2021 Jan 8;6(1):7. doi: 10.1038/s41392-020-00435-w.

75. Structure-based design of melanocortin 4 receptor ligands based on the SHU-9119-hMC4R cocrystal structure. [MEMBRANE PROTEIN]

Martin C, Gimenez LE, Williams SY, Jing Y, Wu Y, Hollanders C, Van der Poorten O, Gonzalez S, Van Holsbeeck K, Previti S, Lamouroux A, Zhao S, Tourwé D, Stevens RC*, Cone RD*, Ballet S*. J Med Chem. 2021 Jan 14;64(1):357-369. doi: 10.1021/acs.jmedchem.0c01620.

74. Furin cleavage sites naturally occur in coronaviruses. [ANTIVIRUS]

Wu Y*, Zhao S*., Stem Cell Res. 2021 Jan;50:102115. doi: 10.1016/j.scr.2020.102115.

2020

73. Targeted proteomics combined with affinity mass spectrometry analysis reveals antagonist E7 acts as an intracellular covalent ligand of orphan receptor GPR52. [MEMBRANE PROTEIN]

Ma M, Guo S, Lin X, Li S, Wu Y, Zeng Y, Hu Y, Zhao S, Xu F, Xie X, Shui W*. ACS Chem Biol. 2020 Dec 18;15(12):3275-3284. doi: 10.1021/acschembio.0c00867.

72. A unique hormonal recognition feature of the human glucagon-like peptide-2 receptor. [MEMBRANE PROTEIN]

Sun W, Chen LN, Zhou Q, Zhao LH, Yang D, Zhang H, Cong Z, Shen DD, Zhao F, Zhou F, Cai X, Chen Y, Zhou Y, Gadgaard S, van der Velden WJC, Zhao S, Jiang Y, Rosenkilde MM, Xu HE*, Zhang Y*, Wang MW*. Cell Res. 2020 Dec;30(12):1098-1108. doi: 10.1038/s41422-020-00442-0.

71. Structural basis for activation of the growth hormone-releasing hormone receptor. [MEMBRANE PROTEIN]

Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D*, Xu HE*, Zhang Y*, Wang MW*. Nat Commun. 2020 Oct 15;11(1):5205. doi: 10.1038/s41467-020-18945-0.

70. Catalytic-state structure and engineering of streptococcus thermophilus Cas9. [ENZYME FUNCTION AND MECHANISM]

Zhang Y, Zhang H, Xu X, Wang Y, Chen W, Wang Y, Wu Z, Tang N, Wang Y, Zhao S, Gan J*, Ji Q*. Nat Catal. 2020 Oct;3:813-823. doi: 10.1038/s41929-020-00506-9.

69. Structural basis of CXC chemokine receptor 2 activation and signalling. [MEMBRANE PROTEIN]

Liu K, Wu L, Yuan S, Wu M, Xu Y, Sun Q, Li S, Zhao S, Hua T*, Liu ZJ*. Nature. 2020 Sep;585(7823):135-140. doi: 10.1038/s41586-020-2492-5. Epub 2020 Jul 1. PMID: 32610344.

68. Virus disinfection from environmental water sources using living engineered biofilm materials. [ANTIVIRUS]

Pu J, Liu Y, Zhang J, An B, Li Y, Wang X, Din K, Qin C, Li K, Cui M, Liu S, Huang Y, Yang Y, Lv Y, Huang J, Cui Z, Zhao S, Zhong C*. Adv Sci. 2020 May 22;7(14):1903558. doi: 10.1002/advs.201903558. PMID: 32714744; PMCID: PMC7375245.

67. Design and synthesis of bitopic 2-Phenylcyclopropylmethylamine (PCPMA) derivatives as selective dopamine D3 receptor ligands. [MEMBRANE PROTEIN]

Tan L, Zhou Q, Yan W, Sun J, Kozikowski AP, Zhao S, Huang XP*, Cheng J*. J Med Chem. 2020 May 14;63(9):4579-4602. doi: 10.1021/acs.jmedchem.9b01835.

66. Targeted Oxidation Strategy (TOS) for potential inhibition of coronaviruses by disulfiram — a 70-year old anti-alcoholism drug. [ANTIVIRUS]

Xu L, Tong J, Wu Y, Zhao S*, Lin BL*. ChemRxiv. 2020 May 3. doi: 10.1101/710673v1. Published version on Biophys Chem (hhttps://www.sciencedirect.com/science/article/pii/S0301462221000922, Ref 83).

65. Determination of the Melanocortin-4 Receptor Structure Identifies Ca2+ as a Cofactor for Ligand Binding. [MEMBRANE PROTEIN]

Yu J, Gimenez LE, Hernandez CC, Wu Y, Wein A, Han GW, McClary K, Mittal S, Burdsall K, Wu L, Stevens SN, Peisley A, Williams SY, Millhauser GL, Zhao S, Cone RD*, Stevens RC*. Science. 2020 Apr 24;368(6489):428-433. doi: 10.1126/science.aaz8995.

64. Novel variants in GDF9 gene affect promoter activity and litter size in mongolia sheep.

Tong B*,Wang J, Cheng Z,Liu J, Wu Y, Li Y, Bai C, Zhao S, Yu H, Li G., Genes. 2020 Mar 30;11(4):E375. doi: 10.3390/genes11040375.

63. The proline synthesis enzyme P5CS forms cytoophidia in drosophila. [ENZYME FUNCTION AND MECHANISM]

Zhang B, Tastan OY, Zhou X, Guo C.-J, Liu X, Thind A, Hu H.-H, Zhao S, Liu JL*. J Genet Genomics. 2020 Mar 19;S1673-8527(20)30047-3. doi: 10.1016/j.jgg.2020.02.005.

62. Evaluation of chemical cross-linkers for in-depth structural analysis of G protein-coupled receptors through cross-linking mass spectrometry. [MEMBRANE PROTEIN]

Xia L, Ma Z, Tong J, Tang Y, Li S, Qin S, Lou R, Zhao S, Lei X*, Shui W*. Anal Chim Acta. 2020 Mar 15;1102:53-62. doi: 10.1016/j.aca.2019.12.036.

61. A novel G protein-biased and subtype-selective agonist for a G protein-coupled receptor discovered from screening herbal extracts. [MEMBRANE PROTEIN]

Zhang B, Zhao S, Yang D, Wu Y, Xin Y, Cao H, Huang X.-P, Cai X, Sun W, Ye N, Xu Y, Peng Y, Zhao S, Liu ZJ, Zhong G*, Wang MW*, Shui W*. ACS Cent Sci. 2020 Feb 26;6(2):213-225. doi: 10.1021/acscentsci.9b01125.

60. The structural study of mutation-induced inactivation of human muscarinic receptor M4. [MEMBRANE PROTEIN]

Wang J, Wu M, Wu L, Xu Y, Li F, Wu Y, Popov P, Wang L, Bai F, Zhao S, Liu ZJ, Hua T*. IUCrJ. 2020 Feb 22;7(Pt 2):294-305. doi: 10.1107/S2052252520000597.

59. Activation and signaling mechanism revealed by cannabinoid Receptor-Gi complex structures. [MEMBRANE PROTEIN]

Hua T*, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ*. Cell. 2020 Feb 20;180(4):655-665.e18. doi: 10.1016/j.cell.2020.01.008.

58. Structural basis of ligand recognition and self-activation of orphan GPR52. [MEMBRANE PROTEIN]

Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J*, Lei M*, Xu F*. Nature. 2020 Feb 19;579(7797):152-157. doi: 10.1038/s41586-020-2019-0.

57. Hybrid spectral library combining DIA-MS data and a targeted virtual library substantially deepens the proteome coverage. [MEMBRANE PROTEIN]

Lou R, Tang P, Ding K, Li S, Tian C, Li Y, Zhao S*, Zhang Y*, Shui W*. iScience. 2020 Feb 12;23(3):100903. doi: 10.1016/j.isci.2020.100903.

56. Identification of a small probe that can be conjugated to proteins by proximity labeling. [MEMBRANE PROTEIN]

Sun W, Huo Y, Mei Y, Zhou Q, Zhao S, Zhuang M*. ACS Chem Biol. 2020 Jan 17;15(1):39-43. doi: 10.1021/acschembio.9b00842.

55. Metabolic reprogramming in cancer is induced to increase proton production. [PATHWAY DOCKING AND GENOME MINING]

Sun H, Zhou Y, Skaro MF, Wu Y, Qu Z, Mao F, Zhao S, Xu Y*. Cancer Res. 2020 Jan 13;80(5):1143-1155. doi: 10.1158/0008-5472.CAN-19-3392.

54. Programmable adenine deamination in bacteria using a Cas9–adenine-deaminase fusion. [ENZYME FUNCTION AND MECHANISM]

Zhang Y, Zhang H, Wang Z, Wu Z, Wang Y, Tang N, Xu X, Zhao S, Chen W*, Ji Q*. J Genet Genomics. 2020 Jan 6;11(6):1657-1664. doi: 10.1039/c9sc03784e.

2019

53. Common activation mechanism of class A GPCRs. [MEMBRANE PROTEIN]

Zhou Q, Yang D, Wu M, Guo Y, Guo W, Zhong L, Cai X, Dai A, Jang W, Shakhnovich EI, Liu ZJ, Stevens RC, Lambert NA, Babu MM*, Wang MW*, Zhao S*. eLife. 2019 Dec 19;8. pii: e50279. doi: 10.7554/eLife.50279.

52. Molecular mechanism for ligand recognition and subtype selectivity of α2C adrenergic receptor. [MEMBRANE PROTEIN]

Chen X, Xu Y, Qu L, Wu L, Han GW, Guo Y, Wu Y, Zhou Q, Sun Q, Chu C, Yang J, Yang L, Wang Q, Yuan S, Wang L, Hu T, Tao H, Sun Y, Song Y, Hu L, Liu ZJ, Stevens RC, Zhao S*, Wu D*, Zhong G*. Cell Rep. 2019 Dec 3;29(10):2936-2943.e4. doi: 10.1016/j.celrep.2019.10.11.

51. Structural basis of the diversity of adrenergic receptors. [MEMBRANE PROTEIN]

Qu L, Zhou Q, Xu Y, Guo Y, Chen X, Yao D, Han GW, Liu ZJ, Stevens RC, Zhong G*, Wu D*, Zhao S*. Cell Rep. 2019 Dec 3;29(10):2929-2935.e4. doi: 10.1016/j.celrep.2019.10.088.

50. GPCR allosteric modulator discovery. [MEMBRANE PROTEIN]

Wu Y, Tong J, Ding K, Zhou Q, Zhao S*. Adv Exp Med Biol (book chapter). 2019;1163:225-251. doi: 10.1007/978-981-13-8719-7_10.

49. Inactivity of YGL082W in vitro due to impairment of conformational change in the catalytic center loop. [ENZYME FUNCTION AND MECHANISM]

Lu L, Guo Y, Wang T, Liang L, Zhao S*, Wang F*, Liu L*. Sci China Chem. 2019 Nov;63(2): 237–243. doi: 10.1007/s11426-019-9623-0.

48. Purification, characterization, and application of a high activity 3-ketosteroid-delta1-dehydrogenase from Mycobacterium neoaurum DSM 1381. [ENZYME FUNCTION AND MECHANISM]

Zhang R, Xu X, Cao H, Yuan C, Yuminaga Y, Zhao S, Shi J*, Zhang B*. Appl Microbiol Biotechnol. 2019 Aug;103(16):6605-6616. doi: 10.1007/s00253-019-09988-5.

47. Colocalization strategy unveils an underside binding site in the transmembrane domain of smoothened receptor. [MEMBRANE PROTEIN]

Zhou F, Ding K, Zhou Y, Liu Y, Liu X, Zhao F, Wu Y, Zhang X, Tan Q, Xu F, Tan W, Xiao Y*, Zhao S*, Tao H*. J Med Chem. 2019 Nov 14;62(21):9983-9989. doi: 10.1021/acs.jmedchem.9b00283.

46. Disulfide‐Containing Detergents (DCDs) for the structural biology of membrane proteins. [MEMBRANE PROTEIN]

Xue D, Xu T, Wang H, Wu M, Yuan Y, Wang W, Tan Q, Zhao F, Zhou F, Hu T, Jiang Z, Liu ZJ, Zhao S, Liu D, Wüthrich K*, Tao H*. Chemistry. 2019 Sep 6;25(50):11635-11640. doi: 10.1002/chem.201903190.

45. Universal activation mechanism of class A GPCRs. [MEMBRANE PROTEIN]

Zhou Q, Yang D, Wu M, Guo Y, Guo W, Zhong L, Cai X, Dai A, Jang W, Shakhnovich EI, Liu ZJ, Stevens RC, Lambert NA, Babu MM*, Wang MW*, Zhao S*. bioRxiv. 2019 Jul 22. doi: 10.1101/710673. Published version on eLife (https://elifesciences.org/articles/50279, Ref 53).

44. The structure-based traceless specific fluorescence labeling of the smoothened receptor. [MEMBRANE PROTEIN]

Xue D, Ye L, Zheng J, Wu Y, Zhang X, Xu Y, Li T, Stevens RC, Xu F, Zhuang M, Zhao S, Zhao F*, Tao H*. Org Biomol Chem. 2019 Jun 26;17(25):6136-6142. doi: 10.1039/c9ob00654k.

43. Molecular mechanism of acetate transport through the acetate channel SatP. [MEMBRANE PROTEIN]

Wu M, Sun L, Zhou Q, Peng Y, Liu ZJ, Zhao S.*, J Chem Inf Model. 2019 May 28;59(5):2374-2382. doi: 10.1021/acs.jcim.8b00975.

42.Advancing chemokine GPCR structure based drug discovery. [MEMBRANE PROTEIN]

Zhao S, Wu B, Stevens RC. Structure. 2019 Mar 5;27(3):405-408. doi: 10.1016/j.str.2019.02.004.

41. Crystal structure of the human cannabinoid receptor CB2. [MEMBRANE PROTEIN]

Li X, Hua T, Vemuri K, Ho JH, Wu Y, Wu L, Popov P, Benchama O, Zvonok N, Locke K, Qu L, Han GW, Iyer M, Cinar R, Coffey N, Wang J, Wu M, Katritch V, Zhao S, Kunos G, Bohn L, Makriyannis A, Stevens RC, Liu ZJ*. Cell. 2020 Feb 20;180(4):655-665.e18. doi: 10.1016/j.cell.2018.12.011.

2018

40. Examination of the deubiquitylation site selectivity of USP51 by using chemically synthesized ubiquitylated histones. [ENZYME FUNCTION AND MECHANISM]

Ai H, Guo Y, Sun D, Liu S, Qi Y, Guo J, Qu Q, Gong Q, Zhao S*, Li J*, Liu L*. ChemBioChem. 2019 Jan 18;20(2):221-229. doi: 10.1002/cbic.201800432.

39. Filamentation of asparagine synthetase in Saccharomyces cerevisiae. [ENZYME FUNCTION AND MECHANISM] .

Zhang S, Ding K, Shen Q, Zhao S, Liu J*. PLoS Genet., 2018 Oct 26;14(10):e1007737. doi: 10.1371/journal.pgen.1007737.

38. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole. [ENZYME FUNCTION AND MECHANISM]

Liu D, Wei Y, Liu X, Zhou Y, Jiang L, Yin J, Wang F, Hu Y, Ankanahalli N; Nanjaraj Urs; Liu Y, Ee Lui Ang; Zhao S*, Zhao H*, Zhang Y*. Nat Commun. 2018 Oct 11, doi: 10.1038/s41467-018-06627-x.

37. Molecular basis for the final oxidative rearrangement steps in chartreusin biosynthesis. [ENZYME FUNCTION AND MECHANISM]

Wang Y, Zhang B, Zhu J, Yang C, Guo Y, Liu C, Liu F, Huang H, Zhao S, Liang Y, Jiao R, Tan R, Ge H., J Am Chem Soc. 2018 Aug 29;140(34):10909-10914. doi: 10.1021/jacs.8b06623.

36. Crystal structure of the Frizzled 4 receptor in a ligand-free state. [MEMBRANE PROTEIN]

Yang S, Wu Y, Xu T, de Waal PW, He Y, Pu M, Chen Y, DeBruine ZJ, Zhang B, Zaidi SA, Popov P, Guo Y, Han G, Lu Y, Suino-Powell K, Dong S,, Harikumar KG, Miller LJ, Katritch V, Xu HE, Shui W, Stevens RC, Melcher K, Zhao S, Xu F*. Nature. 2018 Aug;560(7720):666-670. doi: 10.1038/s41586-018-0447-x.

35. Salvianolic acids from antithrombotic traditional chinese medicine danshen are antagonists of human P2Y₁ and P2Y₁₂ receptors. [MEMBRANE PROTEIN]

Liu X, Gao Z, Wu Y, Stevens RC, Jacobson KA, Zhao S*. Sci Rep. 2018 May 24;8(1):8084. doi: 10.1038/s41598-018-26577-0.

34. Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation. [Protein-protein Interaction]

Shang W, Jiang Y, Boettcher M, Ding K, Mollenauer M, Liu Z, Wen X, Liu C, Hao P, Zhao S, McManus M, Wei L*, Weiss A*, Wang H*. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4051-E4060. doi: 10.1073/pnas.1801340115.

33. Intrinsically disordered proteins link alternative splicing and post-translational modifications to complex cell signaling and regulation. [IDP, MEMBRANE PROTEIN]

Zhou J, Zhao S*, Keith Dunker A*, J Mol Biol. 2018 Apr 4. pii: S0022-2836(18)30179-7. doi: 10.1016/j.jmb.2018.03.028.

32. Succinate-acetate permease from Citrobacter koseri is an anion channel that unidirectionally translocates acetate. [MEMBRANE PROTEIN]

Qiu B, Xia B, Zhou Q, Lu Y, He M, Hasegawa K, Ma Z, Zhang F, Gu L, Mao Q, Wang F, Zhao S, Gao Z*, Liao J*. Cell Res. Mar 27, 2018. doi: 10.1038/s41422-018-0032-8.

31. Identification of natural products as novel ligands for the human 5-HT2C receptor. [MEMBRANE PROTEIN]

Peng Y, Zhao S, Wu Y, Cao H, Xu Y, Liu X, Shui W, Cheng J, Zhao S, Shen L, Ma J, Quinn RJ, Stevens RC, Zhong G*, Liu ZJ*. Biophysics Reports. 2018;4(1):50-61. doi:10.1007/s41048-018-0047-1.

30. High-throughput identification of G protein-coupled receptor modulators through affinity mass spectrometry screening. [MEMBRANE PROTEIN]

Qin S, Meng M, Yang D, Bai W, Lu Y, Peng Y, Song G, Wu Y, Zhou Q, Zhao S, Huang X.-P, McCorvy JD, Cai X, Dai A, Roth B, Hanson MA, Liu ZJ, Wang MW*, Stevens RC, Shui W*. Chem Sci. 2018 Feb 20;9(12):3192-3199. doi:10.1039/C7SC04698G.

29. 5-HT2C receptor structures reveal the structural basis of GPCR polypharmacology. [MEMBRANE PROTEIN]

Peng Y, McCorvy JD, Harpsøe K, Lansu K, Yuan S, Popov P, Qu L, Pu M, Che T, Nikolajsen LF, Huang X.-P, Wu Y, Shen L, Bjørn-Yoshimoto WE, Ding K, Wacker D, Han GW, Cheng J, Katritch V, Jensen AA, Hanson MA, Zhao S, Gloriam DE, Roth BL*, Stevens RC*, Liu ZJ*. Cell. 2018 Feb 8;172(4):719-730.e14. doi: 10.1016/j.cell.2018.01.001.

28. Prediction of enzymatic pathways by integrative pathway mapping. [PATHWAY DOCKING AND GENOME MINING]

Calhoun S, Korczynska M, Wichelecki DJ, San Francisco B, Zhao S, Rodionov DA, Vetting MW, Al-Obaidi NF, Lin H, O'Meara MJ, Scott DA, Morris JH, Russel D, Almo SC, Osterman AL, Gerlt JA*, Jacobson MP*, Shoichet BK*, Sali A*. eLife. 2018 Jan 29;7. pii: e31097. doi: 10.7554/eLife.31097.

2017

27. Structural insights into the committed step of bacterial phospholipid biosynthesis. [MEMBRANE PROTEIN] [ENZYME FUNCTION AND MECHANISM]

Li Z, Tang Y, Wu Y, Zhao S, Bao J, Luo Y, Li D*. Nat Commun. 2017 Nov 22;8(1):1691. doi: 10.1038/s41467-017-01821-9.

26. Crystal structures of agonist-bound human cannabinoid receptor CB1. [MEMBRANE PROTEIN]

Hua T, Vemuri K, Nikas S, Laprairie RB, Wu Y, Qu L, Pu M, Korde A, Shan J, Ho J. H, Han G. W, Ding K, Li X, Liu H, Hanson M. A, Zhao S*, Bohn L. M*, Makriyannis, A*, Stevens, R. C, Liu, Z. J*. Nature. 2017 Jul 27;547(7664):468-471. doi: 10.1038/nature23272.

25. A novel lid-covering peptide inhibitor of nicotinic acetylcholine receptors derived from αD-Conotoxin GeXXA. [MEMBRANE PROTEIN]

Yang L, Tae H.-S, Fan Z, Shao X, Xu S, Zhao S, Adams D*, Wang C*. Mar Drugs. 2017 Jun 5;15(6). pii: E164. doi: 10.3390/md15060164.

24. Structural basis for apelin control of the human apelin receptor. [MEMBRANE PROTEIN]

Ma Y, Yue Y, Ma Y, Zhang Q, Zhou Q, Song Y, Shen Y, Li X, Ma X,Li C, Hanson MA, Han GW, Sickmier EA, Swaminath G, Zhao S, Stevens RC, Hu LA, Zhong W, Zhang M,Xu F*. Structure. 2017 Jun 6;25(6):858-866.e4. doi: 10.1016/j.str.2017.04.008.

23. Human GLP-1 receptor transmembrane domain structure in complex with allosteric modulators. [MEMBRANE PROTEIN]

Song G, Yang D, Wang Y, de Graaf C, Zhou Q, Jiang S, Liu K, Cai X, Dai A, Lin G, Liu D, Wu F, Wu Y, Zhao S, Ye L, Han GW, Lau J, Wu B, Hanson MA, Liu ZJ*, Wang MW*, Stevens RC*. Nature. 2017 Jun 8;546(7657):312-315. doi: 10.1038/nature22378.

22. Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand. [MEMBRANE PROTEIN]

Zhang X, Zhao F, Wu Y, Yang J, Han GW, Zhao S, Ishchenko A, Ye L, Lin X, Ding K, Dharmarajan V, Griffin PR, Gati C, Nelson G, Hunter MS, Hanson MA, Cherezov V, Stevens RC, Tan W*, Tao H*, Xu F*. Nat Commun. 2017 May 17;8:15383. doi: 10.1038/ncomms15383.

21. A structurally guided dissection-then-evolution strategy for ligand optimization of smoothened receptor. [MEMBRANE PROTEIN]

Ye L, Ding K, Zhao F, Liu X, Wu Y, Liu Y, Xue D, Zhou F, Zhang X, Stevens RC, Xu F, Zhao S*, Tao H*. MedChemComm. 2017,8, 1332-1336.doi: 10.1039/C7MD00104E.

20. Exploring the mutational robustness of nucleic acids by searching genotype neighborhoods in sequence space.

Zhou Q, Sun X, Xia X, Fan Z, Luo Z, Zhao S, Shakhnovich E*, Liang H*. J Phys Chem Lett. 2017 Jan 19;8(2):407-414. doi: 10.1021/acs.jpclett.6b02769.

2016

19. Crystal structure of the human cannabinoid receptor CB1. [MEMBRANE PROTEIN]

Hua T, Vemuri K, Pu M, Qu L, Han GW, Wu Y, Zhao S, Shui W, Li S, Korde A, Laprairie RB, Stahl EL, Ho JH, Zvonok N, Zhou H, Kufareva I, Wu B, Zhao Q, Hanson MA, Bohn LM*, Makriyannis A*, Stevens RC*, Liu ZJ*. Cell. 2016 Oct 20;167(3):750-762.e14. doi: 10.1016/j.cell.2016.10.004.

18. Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars. [PATHWAY DOCKING AND GENOME MINING]

Zhang XS, Carter MS, Vetting MW, Francisco BS, Zhao S, Al-Obaidi NF, Solbiati JO, Thiaville JJ, de Crecy-Lagard V, Jacobson MP, Almo SC, Gerlt JA*. Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4161-9. doi: 10.1073/pnas.1605546113.

2015

17. Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes. [PATHWAY DOCKING AND GENOME MINING]

Vetting MW#; Al-Obaidi N#; Zhao S#; San Francisco B#; Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ*, Osterman AL*, Cronan JE*, Jacobson MP*, Gerlt JA*, Almo SC*. Biochemistry. 2015 Jan 27;54(3):909-31. doi: 10.1021/bi501388y.

16. A unique cis-3-hydroxy-l-proline dehydratase in the enolase superfamily. [PATHWAY DOCKING AND GENOME MINING]

Zhang X, Kumar R, Vetting MW, Zhao S, Jacobson MP, Almo SC, Gerlt JA*. J Am Chem Soc. 2015 Feb 4;137(4):1388-91. doi: 10.1021/ja5103986.

2014

15. Leveraging structure for enzyme function prediction: methods, opportunities, and challenges. [PATHWAY DOCKING AND GENOME MINING]

Jacobson MP*, Kalyanaraman C, Zhao S, Tian B., Trends Biochem Sci. 2014 Aug;39(8):363-71. doi: 10.1016/j.tibs.2014.05.006.

14. Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks. [PATHWAY DOCKING AND GENOME MINING]

Zhao S, Sakai A, Zhang X, Vetting MW, Kumar R, Hillerich B, San Francisco B, Solbiati J, Steves A, Brown S, Akiva E, Barber A, Seidel R. D, Babbitt PC, Almo SC*, Gerlt JA*, Jacobson MP*. eLife. 2014 Jun 30;3. doi: 10.7554/eLife.03275.

13. Prediction and biochemical demonstration of a catabolic pathway for the osmoprotectant proline betaine. [PATHWAY DOCKING AND GENOME MINING]

Kumar R, Zhao S, Vetting MW, Wood BM, Sakai A, Cho K, Solbiati J, Almo SC, Sweedler JV, Jacobson MP, Gerlt JA*, Cronan JE*. mBio. 2014 Feb 11;5(1):e00933-13. doi: 10.1128/mBio.00933-13.

2013

12. Discovery of new enzymes and metabolic pathways by using structure and genome context. [PATHWAY DOCKING AND GENOME MINING]

Zhao S, Kumar R, Sakai A, Vetting MW, Wood BM, Brown S, Bonanno JB, Hillerich BS, Seidel RD, Babbitt PC, Almo SC, Sweedler JV*, Gerlt JA*, Cronan JE*, Jacobson MP*. Nature. 2013 Oct 31;502(7473):698-702. doi: 10.1038/nature12576.